Insect Histology

Insect Histology: Practical Laboratory Techniques
Pedro Barbosa, Deborah Berry & Christina K. Kary
1st Edition
368 pages
ISBN-13: 978-1444336962
ISBN-10: 1444336967

This helpful guide is self-contained, holding all of the information you will need for the preparation of histological slides of all types of insect tissues. It explains the essential procedures for their preparation, providing step-by-step information on each phase, for example, on fixation, dehydrating, clearing, embedding, and staining.
The laboratory manual explains the cutting-edge methodology carried out today, and provides a thorough collection of both historically established and modern insect histology techniques side-by-side. It is the first book to present these various techniques appropriate to insects.
Key issues covered include:
- The use of genetic markers in insect histology;
- Important approaches for the preparation of tissues and
organs for SEM and TEM Fluorescence;
- New immunohistochemical techniques;
- Histological problems encountered
in insect tissues such as sclerotized chitin, yolk-laden eggs, chromosomes,
genitalia, etc., and how to deal with them.
Photographs show the likely results of procedures, components of the process, and advantages of the use of particular approaches or compounds. As a comprehensive resource, this book is for students and researchers in entomology, systematics, developmental biology, insect cell biology, and morphology, wanting a clear introduction to the procedures for the histological display of insect tissues.



Table of Contents

Preface ix
Acknowledgements xi
Introduction xiii
1 Problems of sclerotized chitin: Softening insect cuticle 1
2 Fixation 21
3 Dehydrating, clearing, and embedding 54
4 Staining 79
5 Immunohistochemical techniques 119
6 Use of genetic markers in insect histology 146
7 Fluorescence 171
8 Mounting 194
9 Preparation of whole mounts 208
10 Preparation of whole mounts for staining 231
11 Preparation of genitalia, mouthparts and other body parts 246
12 Preparation of chromosomes 258
13 Preparation of other specific insect organs and tissues 290
Appendix Dissecting fluids and saline solutions 325
Index 333